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yodB [2017-12-15 18:22:34]
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yodB [2017-12-15 18:22:34]

MarR/DUF24 family transcription repressor of azoR1, catD-catE and yodC expression
locus
BSU19540
pI
4.74
mw
12.71 kDa
protein length
112 aa Sequence Blast
gene length
336 bp Sequence Blast
function
regulation of quinone and diamide detoxification
product
MarR/DUF24 family transcription repressor
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

    • Gene

    Coordinates
    2,127,345 → 2,127,683

    Phenotypes of a mutant

  • increased resistance to catechol, quinones and diamide [Pubmed|20639328]

    • The protein

    Protein family

  • [[MarR/DUF24 family]]

    • Paralogous protein(s)

  • [protein|5B9C4932B2E76D6B8F6BDB640D1ADEE28F488FAD|CatR], [protein|E757C4B329A99E3D0C70C77437DF4DDA300CA5E7|YdzF]

    • Modification

  • redox-controlled by intersubunit disulfide formation of Cys6 and Cys101' in response to quinones and diamide

    • Effectors of protein activity

  • responds distinctly to diamide or methyl-p-benzoquinone [Pubmed|27531959]

    • Structure

  • [PDB|5HS7] (reduced form) [Pubmed|27531959]
  • [PDB|5HS8] (diamide-treated form) [Pubmed|27531959]
  • [PDB|5HS9] (quinone-bound form) [Pubmed|27531959]

    • Expression and Regulation

    Operons

    genes
    [gene|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|yodB]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|YodB]: repression, in [regulon|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|YodB regulon]
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    Biological materials

    Mutant

  • MGNA-B433 (yodB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1432 NBRP B. subtilis, Japan]
  • BKE19540 (Δ[gene|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|yodB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGTCTTCATCCCTTCA, downstream forward: _UP4_TAAGGAAAGCGGCACCTGAA
  • BKK19540 (Δ[gene|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|yodB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGTCTTCATCCCTTCA, downstream forward: _UP4_TAAGGAAAGCGGCACCTGAA

    • Labs working on this gene/protein

  • [SW|Haike Antelmann],University of Greifswald, Germany

    • References

    Reviews

  • 18282125,20626317

    • Original Publications

  • 17158660,17407181,18208493,20639328,20652907,21749987,27531959